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Electrophoresis
SPIFE 4000
Helena SPIFE 4000

Total Automation for IFEs and SPEs

Yes, you really can just load the sample tubes, press go, and return for your printed IFE and SPE results! SPIFE 4000 eliminates ALL manual steps for these assays ? there’s no sample application, no stains or antisera to add, no gel transfers, no dilutions to perform, no gel blocks to remove, no tech-to-tech variability ? just load and walk away. A full day’s mixed run of SPEs and IFEs takes about 30 minutes of tech time for set up, then frees the tech to perform other tasks. In a single 8-hour shift, SPIFE 4000 can turn 100 IFEs or 756 SPEs. With SPIFE 4000’s hands-off approach, a laboratory could reasonably expect to be able to run 24 hours per day 7 days a week
Product Detail

 

SPIFE 4000 FEATURES
? Total IFE & SPE automation
? Full host connectivity
? Positive ID
? Primary tube sampling
? Auto dilutions
? Auto gel transfers
? Auto antisera application
? Auto stain & destain
? Auto densitometry

Auto Sampling for SPE & IFE -

? Liquid level sensing
? Auto dilutions for immunofixation
? Positive ID from tube to gel to final report

Auto Applicator and Electrophoresis -

? Auto sample application to gels
? Application system unaffected by triglycerides or large proteins
? Peltier-driven temperature controlled chamber   

Integrated Auto Scanning -

? Automatic setting of scan parameters
? Full editing and interpretation capabilities
? Final results can be printed and/or exported to LIS

 

 SPIFE 4000

Cat. No. Item
1620 SPIFE 4000, 110V
1621 SPIFE 4000, 220V
1622 SPIFE 4000, 100V/50Hz
1623 SPIFE 4000, 100V/60Hz
1625 SPIFE 4000 Replacement Electrodes
1630 SPIFE 4000 Cassettes (10/pkg)
1635 SPIFE 4000 Rigid Antisera Template
1636 SPIFE 4000 Serum Separator Sleeves, 28/pkg
2304 SPIFE 4000 IFE Antisera Pipette Tips, 96/pkg
2305 SPIFE 4000 IFE Sample Trays, 10/pkg
2307 SPIFE 4000 Maintenance Blotters, 20/pkg
2308 SPIFE 4000 Gel Staging Lid
2309 SPIFE 4000 Contact Sheets (5/pkg)
2315 SPIFE 4000 White Sample Trays, 10/pkg
2316 SPIFE 4000 Blue Sample Trays, 10/pkg
2319 SPIFE 4000 Applicator Blades
1033 Neutral Density Densitometer Control for SPIFE 4000
9136 Service Manual for SPIFE


SPIFE4000 IFE

SPIFE 4000 automatically performs all the steps for immunofixation.
Auto Dilutions ? the sample handler performs all the needed dilutions and applies samples to gels
Auto Antisera - Antisera are automatically applied to gels. Affinity and avidity of the antisera are balanced for best sensitivity with the least amount of blush.
Auto Press and Blot ? Assures clear backgrounds, before moving into the auto stainer
Throughput ? Four profiles per gel; up to 100 IFEs per 8 hours

Cat. No. Item
2300 40 tests
2301 400 tests

 

SPIFE4000 SPE

No protein retention ? application system is unaffected by triglycerides or large proteins.
Active cooling ? excellent split-beta separation with no degradation
Great results ? resolution and presentation are excellent
Throughput ? 28 samples per gel; up to 756 per 8 hours

Cat. No. Item
2310 280 tests
2311 2800 tests

 

Alkaline Hb

SPIFE 4000 can perform alkaline hemoglobin separation for eight samples per gel, with three gels per run.

? Aids in identifying hemoglobinopathies
? Stable in storage with acid blue stain
? Automated from sample application to finished gel

Cat. No. Item
2320 SPIFE 4000 Alkaline Hb Kit (80 tests)
2324 SPIFE 4000 Alkaline Hb Cassettes and Electrodes
1359 REP Prepper

 

Acid Hb
Separate up to 14 acid hemoglobin samples per gel and up to three gels per run on SPIFE 4000.

? Aids in identifying hemoglobinopathies
? Stable in storage with acid blue stain
? Excellent separations

Cat. No. Item
2322 SPIFE 4000 Acid Hb Kit (140 tests)
2315 SPIFE 4000 White Sample Trays (10/pkg)

 

Hb IEF
SPIFE 4000 automates isolectric focusing of hemoglobins on up to 14 samples from sample application to finished gel.

? Produces crisp, sharply resolved hemoglobin bands
? Aids in distinguishing hemoglobin variants
? Final results in 75 minutes

Cat. No. Item
2325 SPIFE 4000 Hb IEF (140 tests)
2327 SPIFE 4000 Hb IEF Cassettes and Electrodes

 

Performance Specifications

Tests:
Serum Proteins and IFE

Media Types:
Agarose and cellulose acetate gels

Maximum Sample Size:
5 x 5 in. (12.7 x 12.7 cm)

Input Power:
120 VAC, 60 Hz, 1000 Watts
230 VAC, 50 Hz, 1000 Watts

Leakage Current:
< 3.5 mA. sinusoidal

Dimensions: Height x Width x Depth:
SPIFE 4000 Instrument & Fluids Table:
60 x 79 x 32 (152.4 x 200.7 x 81.3 cm)

Computer, Monitor, and Keyboard:
22 x 18 x 24 in. (56 x 48 x 61 cm)

Scanner:
< or equal to 8 x 13 x 22 in. (20 x 33 x 56 cm)

Printer:
10 x 14 x 14 in. (26 x 36 x 36 cm)

Total Weight: 350 lbs (158.8 kg)

Environment: 15° to 27°C (59° to 80°F)

Instrument Operating Environment:

Ambient Temperature Range -
15° to 27°C (59° to 80°F)

Altitude tested up to 2000 meters

Complete specifications for the scanner, monitor and printer can be found in their respective owner's guide.

Densitometer Specifications

Scanning Parameters:
Gain: Automatic or Manual
Over range: > 100% of full scale
Optics Mode:
Visible Transmittance (white light)

Aperture Sizes (mm):
1....... 1 x .1
2....... 2 x .2
3....... 3 x .3
4....... 4 x .4
5....... 6 x .6
6....... 7 x .7
7....... 8 x .8
8....... 2.5 x .25
9....... 5 x .1
10......5 x .25
11..... 5 x .5
12 .....12 x 12

Scan Length:
Variable from 0.01 to 221.5 mm

Zeroing:
Automatic

Light Source:
Cold Cathode Fluorescent (white light)

Light Detectors:
CCD Array

Optical Density Linearity:
0.05 to 2.3

 

Operating System:

*Windows is a registered trademark of Microsoft Corporation in the United States and other countries.
Microsoft ? Windows? XP

Electrophoresis Timer Range: 1 sec. to 90 min. in 1 second increments
Chamber Temperature Range: 10° to 30°C
Pre-Drying Temperature Range: 30° to 62°C
Final Drying Temperature Range: 30° to 65°C (86° to 149°F)
Fuses: F1, F2 15A/250V slow blow
Pump System: 2 peristaltic pumps, 1 syringe pump
Test Tubes: 13-16 mm diameter, 75-100 mm height (Minimum sample volume 500uL)

Bar Codes:

Type

Character Density
Minimum Bar Width

Ratio
Thin to Thick

Maximum Characters
Bar Width

Code 3 of 6 (Code 9)
(alphanumeric)

0.0075

3:1

10

Codabar (numeric)

0.0098

n/a

10

Interleaved 2 of 5
(numeric)

0.0075

3:1

20

UPC - 12 (numeric)

0.014

n/a

13

EAN, L reduced
(numeric)

0.014

n/a

13

CODE 128
(alphanumeric)

0.0075

3:1

25

 

spife 4000 spec graphic

 

Electrophoresis Instrument and LIS Interfacing:

Ports: 3-wire RS232, no hardware handshaking

Programmable Baud Rate:

1200, 2400, 4800, 9600, 14400, 19200

Programmable Data Bits:

7, 8

Programmable Stop Bits:

1, 2

Programmable Parity:

None, Odd, Even

Programmable Protocol:

None, XON-XOFF, ASTM